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. 2020 Nov 25;19(10):1586–1601. doi: 10.1074/mcp.RA120.002163

Table II.

Average ion intensities DCBLD1 and DCBLD2 phosphopeptides. Average ion intensities of all quantifiable singly phosphorylated peptides identified in label-free quantification studies for A, DCBLD1 and B, DCBLD2 are tabulated. Intensities of quantifiable singly phosphorylated peptides (S/N >10) were normalized to unmodified loading control peptides (Table I) for comparison of relative phosphopeptide intensities across conditions. Phosphorylated species that were not resolve chromatographically were quantified together, indicated by multiple site localizations with one average intensity. These intensities were used to quantify relative changes in phosphopeptide intensities represented in the heat map in Fig. 1

A
Site(s) Average normalized intensity
DCBLD1 DCBLD1 + ABL
S488 4.14E + 07 9.85E + 06
S513 6.52E + 07 1.29E + 08
Y540 8.16E + 07
S556 2.03E + 08 2.33E + 08
Y578 3.22E + 08
Y589 9.91E + 07
Y600 3.65E + 07 7.12E + 08
T602 2.04E + 07
Y621 9.27E + 06 9.12E + 08
S633, S635, S636, S640 8.83E + 08 1.90E + 08
Y652 4.57E + 06 2.28E + 08
S657, Y665 5.04E + 07
S672 2.16E + 06
S963, S695, Y696, S697 3.89E + 06 9.39E + 08
B
Site(s) Average Normalized Intensity
DCBLD2 DCBLD2 + ABL DCBLD2 + FYN
Y565 1.09E + 07 8.35E + 07
Y569 4.38E + 07
T593, Y597, S598, S599, S600 8.42E + 07 8.71E + 07
S618 5.36E + 07 1.16E + 07
Y621 1.45E + 08 4.71E + 07
Y649 1.21E + 08 1.39E + 07
Y655 3.89E + 06 5.21E + 06
S657 4.35E + 06 3.73E + 06 5.96E + 06
Y663, Y666 1.17E + 06 5.22E + 07
Y677 1.58E + 07 3.40E + 06
T679 2.00E + 06
Y715 8.91E + 07 5.29E + 07
S724, S727 9.58E + 07 1.16E + 07 4.70E + 07
Y750 6.14E + 07 2.02E + 07
T756, S760 7.63E + 05