Fig. 1.

DOC induces a large proteome shift in C. jejuni NCTC11168 and influences phenotypes associated with virulence.A, Volcano plot for cells grown in 0.1% DOC compared with control; x axis represents averaged Log₂(DOC/control), y axis represents log₁₀(p value). Significantly differentially abundant proteins are highlighted in red (p < 0.05); B, Correlation plot based on n-fold changes observed in the discovery set (n-fold DDA_TMT) and validation set (n-fold DIA-SWATH-MS), Pearson correlation r = 0.8213 (p < 0.0001) determined from 1104 aligned proteins; C, Heat map of C. jejuni proteins associated with antibiotic resistance and efflux ordered by largest mean n-fold change (label-based discovery; top). Data from each of 4 label-based replicates (n-fold 1–4) and DIA-SWATH-MS validation (‘DIA’ mean of 2 biological replicates [DIA n-fold 1–2]) are shown. Values are gray where the protein was not identified by DIA-SWATH-MS (* denotes proteins significantly altered in abundance); D, Increased motility associated with growth in 0.1% DOC based on colony diameter in mm on semi-solid agar (*** p < 0.001); E, Antibiotic resistance assays for (i) polymyxin B, (ii) ciprofloxacin (** p < 0.005, * p < 0.05).