Fig. 8.

Cj0025c is a TcyP-like Cys-Cys transport protein.A, Selenocystine inhibition assay (i) Se-Cys-Cys inhibits WT C. jejuni and 0.1% DOC increases inhibition consistent with increased abundance of Cj0025c under 0.1% DOC growth (Fig. 3B), whereas Δcj0025c is only slightly inhibited and 0.1% DOC does not influence this phenotype (*** p < 0.001, n.s. not significant), (ii) representative Se-Cys-Cys inhibition plates (upper) C. jejuni WT and (lower) Δcj0025c; B, Provision of an alternative sulfur source (2 mm thiosulfate [TS]) (i) restores Δcj0025c motility toward WT levels, (ii) representative motility plate assay (upper) Δcj0025c, (lower) Δcj0025c + 2 mm TS (*** p < 0.001).