Table V.
All GAPDH peptides quantified in this study
| Protein | Peptide sequence | Modifications | Fold change (treated:untreated) | Adjusted p-value | Manual localization of oxidative modification |
|---|---|---|---|---|---|
| Escherichia coli GAPDH (Accession number: P0A9B2) | [K].YAGQDIVSNASCTTNCLAPLAK.[V] | 1xCarbamidomethyl [C16]; 1 ×Trioxidation [C12]; 1 ×TMT6plex [K22]; 1 ×TMT6plex [N-Term] | 20.12 | 5.1E-03 | OK |
| [K].AGIALNDNFVK.[L] | 1xTMT6plex [K11]; 1 ×TMT6plex [N-Term] | 2.02 | 4.7E-03 | N/A | |
| [K].LTGMAFR.[V] | 1xOxidation [M4]; 1 ×TMT6plex [N-Term] | 1.97 | 4.1E-03 | OK | |
| [K].DNTPMFVK.[G] | 1xTMT6plex [K8]; 1 ×TMT6plex [N-Term] | 1.92 | 7.3E-03 | N/A | |
| [K].LTGMAFR.[V] | 1xTMT6plex [N-Term] | 1.90 | 3.9E-03 | N/A | |
| [K].DNTPMFVK.[G] | 1xOxidation [M5]; 1 ×TMT6plex [K8]; 1 ×TMT6plex [N-Term] | 1.88 | 5.8E-03 | OK | |
| [R].GASQNIIPSSTGAAK.[A] | 1xTMT6plex [K15]; 1 ×TMT6plex [N-Term] | 1.86 | 9.5E-03 | N/A | |
| [R].FDGTVEVK.[D] | 1xTMT6plex [K8]; 1 ×TMT6plex [N-Term] | 1.86 | 1.9E-02 | N/A | |
| [K].LVSWYDNETGYSNK.[V] | 1xTMT6plex [K14]; 1 ×TMT6plex [N-Term] | 1.83 | 6.6E-03 | N/A | |
| [K].VGINGFGR.[I] | 1xTMT6plex [N-Term] | 1.79 | 9.1E-03 | N/A | |
| [K].VLPELNGK.[L] | 1xTMT6plex [K8]; 1 ×TMT6plex [N-Term] | 1.71 | 6.6E-03 | N/A | |
| [R].VPTPNVSVVDLTVR.[L] | 1xTMT6plex [N-Term] | 1.52 | 5.7E-03 | N/A | |
| [K].AATYEQIK.[A] | 1xTMT6plex [K8]; 1 ×TMT6plex [N-Term] | 1.52 | 2.2E-02 | N/A | |
| [K].VVMTGPSK.[D] | 1xTMT6plex [K8]; 1 ×TMT6plex [N-Term] | 1.49 | 2.7E-02 | N/A | |
| [R].VPTPNVSVVDLTVRLEK.[A] | 1xTMT6plex [K17]; 1 ×TMT6plex [N-Term] | 1.43 | 2.8E-01 | N/A | |
| [K].VVMTGPSK.[D] | 1xOxidation [M3]; 1 ×TMT6plex [K8]; 1 ×TMT6plex [N-Term] | 1.39 | 2.5E-02 | OK | |
| [K].AAAEGEMK.[G] | 1xOxidation [M7]; 1 ×TMT6plex [K8]; 1 ×TMT6plex [N-Term] | 1.33 | 1.4E-01 | OK | |
| [R].VTAERDPANLK.[W] | 1xTMT6plex [K11]; 1 ×TMT6plex [N-Term] | 1.32 | 1.8E-01 | N/A | |
| [K].GANFDK.[Y] | 1xTMT6plex [K6]; 1 ×TMT6plex [N-Term] | 1.30 | 2.4E-01 | N/A | |
| [K].VLDLIAHISK.[-] | 1xTMT6plex [K10]; 1 ×TMT6plex [N-Term] | 1.28 | 1.4E-01 | N/A | |
| [K].AAAEGEMK.[G] | 1xTMT6plex [K8]; 1 ×TMT6plex [N-Term] | 1.21 | 7.9E-02 | N/A | |
| [K].YAGQDIVSNASCTTNCLAPLAK.[V] | 2xCarbamidomethyl [C12; C16]; 1 ×TMT6plex [K22]; 1 ×TMT6plex [N-Term] | 1.12 | 3.6E-01 | N/A | |
| [K].YAGQDIVSNASCTTNCLAPLAK.[V] | 2xCarbamidomethyl [C12; C16]; 1 ×Oxidation [T13]; 1 ×TMT6plex [K22]; 1 ×TMT6plex [N-Term] | 1.06 | 6.5E-01 | Ambiguous between T13/T14 | |
| [K].DGHLIVNGK.[K] | 1xTMT6plex [K9]; 1 ×TMT6plex [N-Term] | 1.03 | 9.3E-01 | N/A | |
| [K].TVDGPSHK.[D] | 1xTMT6plex [K8]; 1 ×TMT6plex [N-Term] | 0.90 | 7.8E-01 | N/A | |
| Deinoccous radiodurans GAPDH (Accession number: Q9RUP1) | [R].GVEVVAINDLTDNHTLAHLLK.[Y] | 1xTMT6plex [K21]; 1 ×TMT6plex [N-Term] | 1.21 | 1.7E + 00 | N/A |
| [K].KVIITAPAK.[G] | 2xTMT6plex [K1; K9]; 1 ×TMT6plex [N-Term] | 1.19 | 1.1E + 00 | N/A | |
| [R].VLDLPHSDLRR.[A] | 1xTMT6plex [N-Term] | 1.11 | 1.2E + 00 | N/A | |
| [R].IADLVQLVQNK.[G] | 1xTMT6plex [K11]; 1 ×TMT6plex [N-Term] | 1.10 | 1.1E + 00 | N/A | |
| [K].VIDEAFGIEK.[A] | 1xTMT6plex [K10]; 1 ×TMT6plex [N-Term] | 1.09 | 1.3E + 00 | N/A | |
| [R].AAAINIIPTSTGAAK.[A] | 1xTMT6plex [K15]; 1 ×TMT6plex [N-Term] | 1.08 | 1.5E + 00 | N/A | |
| [R].VPTPTGSISDVSVILGR.[D] | 1xTMT6plex [N-Term] | 1.07 | 1.2E + 00 | N/A | |
| [R].VLDLPHSDLR.[R] | 1xTMT6plex [N-Term] | 1.07 | 1.3E + 00 | N/A | |
| [K].AIMTTVHSYTNDQR.[V] | 1xTMT6plex [N-Term] | 1.07 | 1.0E + 00 | N/A | |
| [K].AVSQVYPALK.[G] | 1xOxidation [Y6]; 1 ×TMT6plex [K10]; 1 ×TMT6plex [N-Term] | 1.07 | 1.2E + 00 | OK | |
| [K].AVSQVYPALK.[G] | 1xOxidation [Y6]; 1 ×Carbonyl [V5]; 1 ×TMT6plex [K10]; 1 ×TMT6plex [N-Term] | 1.06 | 1.0E + 00 | OK | |
| [K].AIMTTVHSYTNDQR.[V] | 2xOxidation [M3; N/I]; 1 ×TMT6plex [N-Term] | 1.04 | 1.0E + 00 | Yes M3, Ambiguous on second between Y9-N11 | |
| [K].IQAIAERDPANIK.[W] | 1xTMT6plex [K13]; 1 ×TMT6plex [N-Term] | 1.02 | 1.0E + 00 | N/A | |
| [R].DVTVEEVNNVFR.[E] | 1xTMT6plex [N-Term] | 1.02 | 1.0E + 00 | N/A | |
| [K].AVSQVYPALK.[G] | 1xTMT6plex [K10]; 1 ×TMT6plex [N-Term] | 1.01 | 1.0E + 00 | N/A | |
| [K].AVSQVYPALK.[G] | 1xDioxidation [Y/P]; 1 ×TMT6plex [K10]; 1 ×TMT6plex [N-Term] | 0.99 | 1.0E + 00 | Ambiguous, almost certainly on Y6 | |
| [R].FDGTVEYDESSLTVNGK.[K] | 1xTMT6plex [K17]; 1 ×TMT6plex [N-Term] | 0.95 | 1.0E + 00 | N/A | |
| [K].AIMTTVHSYTNDQR.[V] | 1xOxidation [T/V/M]; 1 ×TMT6plex [N-Term] | 0.92 | 1.1E + 00 | Ambiguous, almost certainly on M3 | |
| [K].FDGTSLR.[V] | 1xTMT6plex [N-Term] | 0.90 | 1.0E + 00 | N/A | |
| [K].VIITAPAK.[G] | 1xTMT6plex [K8]; 1 ×TMT6plex [N-Term] | 0.89 | 1.3E + 00 | N/A | |
| [K].IQAIAER.[D] | 1xTMT6plex [N-Term] | 0.87 | 1.2E + 00 | N/A | |
| Homosapiens GAPDH (Accession number: P04406) | [R].VPTANVSVVDLTCR.[L] | 2.116 | 1.41E-04 | N/A | |
| [K].VIHDNFGIVEGLMTTVHAITATQK.[T] | 1xOxidation [M/T/L] | 1.694 | 1.39E-03 | Ambiguous, almost certainly on M13 | |
| [R].VVDLMAHMASK.[E] | 1.464 | 1.15E-02 | N/A | ||
| [R].VPTANVSVVDLTCR.[L] | 1xCarbamidomethyl [C13] | 1.452 | 5.22E-03 | N/A | |
| [R].VVDLMAHMASKE.[-] | 1.405 | 3.38E-02 | N/A | ||
| [K].LISWYDNEFGYSNR.[V] | 1xDioxidation [W/Y]; 2 ×Oxidation [W4; Y5] | 1.271 | 2.54E-01 | Ambiguous, almost certainly Dioxidation on W4 | |
| [K].LTGMAFR.[V] | 1.259 | 1.88E-01 | N/A | ||
| [K].LVINGNPITIFQERDPSK.[I] | 1.176 | 6.23E-01 | N/A | ||
| [K].VGVNGFGR.[I] | 1.161 | 5.83E-01 | N/A | ||
| [R].VIISAPSADAPMFVMGVNHEK.[Y] | 1.158 | 5.95E-01 | N/A | ||
| [R].VVDLMAHMASKE.[-] | 1xOxidation [L/H/M] | 1.126 | 9.27E-01 | Ambiguous, almost certainly on M5 | |
| [K].IISNASCTTNCLAPLAK.[V] | 2xCarbamidomethyl [C7; C11] | 1.122 | 7.72E-01 | N/A | |
| [K].VIPELNGK.[L] | 1.107 | 8.35E-01 | N/A | ||
| [K].RVIISAPSADAPMFVMGVNHEK.[Y] | 1.069 | 9.89E-01 | N/A | ||
| [R].GALQNIIPASTGAAK.[A] | 1.021 | 1.00E + 00 | N/A | ||
| [K].LVINGNPITIFQER.[D] | 0.98 | 1.00E + 00 | N/A | ||
| [R].VVDLMAHMASK.[E] | 1xOxidation [M/H] | 0.961 | 1.00E + 00 | Ambiguous, almost certainly on M5 | |
| [K].LISWYDNEFGYSNR.[V] | 1xCarbonyl [E8] | 0.935 | 9.89E-01 | OK | |
| [K].LISWYDNEFGYSNR.[V] | 1xOxidation [W4] | 0.882 | 9.28E-01 | OK | |
| [K].WGDAGAEYVVESTGVFTTMEK.[A] | 1xCarbonyl [E7] | 0.879 | 9.45E-01 | OK | |
| [K].AGAHLQGGAK.[R] | 0.865 | 9.27E-01 | N/A | ||
| [R].VIISAPSADAPMFVMGVNHEK.[Y] | 1xDioxidation [F13]; 2 ×Oxidation [M12; M15] | 0.851 | 6.68E-01 | Ambiguous, most likely hydroxylation on both M12/M15 | |
| [K].LISWYDNEFGYSNR.[V] | 0.844 | 6.29E-01 | N/A | ||
| [K].LISWYDNEFGYSNR.[V] | 1xTrioxidation [Y/W] | 0.841 | 9.72E-01 | Ambiguous between W4/Y5 | |
| [K].WGDAGAEYVVESTGVFTTMEK.[A] | 0.811 | 3.95E-01 | N/A | ||
| [K].QASEGPLK.[G] | 0.803 | 3.43E-01 | N/A | ||
| [K].WGDAGAEYVVESTGVFTTMEK.[A] | 1xOxidation [M/Y/E/S] | 0.702 | 2.10E-02 | Ambiguous, almost certainly on M19 | |
| [R].VIISAPSADAPMFVMGVNHEK.[Y] | 1xOxidation [M/F/P] | 0.694 | 1.57E-02 | OK | |
| [K].TVDGPSGK.[L] | 0.617 | 7.53E-03 | N/A | ||
| [K].VIHDNFGIVEGLMTTVHAITATQK.[T] | 0.491 | 1.30E-09 | N/A | ||
| [K].WGDAGAEYVVESTGVFTTMEK.[A] | 1xOxidation [T18]; 1 ×Carbonyl [E7] | 0.491 | 1.77E-01 | Yes E7, Ambiguous hydroxylation on T18 or M19 | |
| [R].VVDLMAHMASK.[E] | 2xOxidation [M5; M8] | 0.474 | 1.14E-04 | OK | |
| [K].GILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVK.[L] | 0.281 | 6.56E-16 | N/A | ||
| [K].IISNASCTTNCLAPLAK.[V] | 1xCarbamidomethyl [C]; 1 ×Dioxidation [C7]; 2 ×Oxidation [S3; S6] | 1000 Gy only | 6.56E-16 | Ambiguous between hydroxylation on S3/S6 and dioxidation on C7 | |
| [K].IISNASCTTNCLAPLAK.[V] | 1xCarbamidomethyl [C11]; 1 ×Trioxidation [C7] | 1000 Gy only | 6.56E-16 | OK | |
*Peptides listed map to the GAPDH protein of each respective organism. Adjusted p-values listed are Benjamini-Hochberg adjusted. Each oxidative modification was assessed by manual inspection of mass spectra, as indicated by the far-right column. Protein accession numbers are from UniProt (85).