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. 2021 Apr 1;12:223. doi: 10.1186/s13287-021-02280-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Characterization of hAECs, detection of the survival hAECs encapsulated in SA-BG, and schematic illustration of the surgical procedure. a The morphology of cultured hAECs was observed under a microscope. Scale bar 100 μm. b Flow cytometry analysis of cell surface markers on hAECs. The isotypes (ISO) of SSEA4 and CD324 were used as negative controls. c Immunostaining images showed the high expression of epithelial marker (CK18) and stem cell marker (TRA-1-60). Scale bar 100 μm. d The fabrication method of SA-BG-loaded hAECs and CM. e Representative live/dead images of hAECs encapsulated in SA-BG at days 1, 7, 10, and 14, respectively. hAECs encapsulated in SA-BG treated with 70% alcohol were positive for PI. Live cells were shown green color and dead cells were red color. Scale bar 100 μm. f Bright field image of hAECs capsulated in SA-BG at day 1 (a) and 14 (b). Scale bar 100 μm. g The percentage of live cells to total cells. h Schematic of the experimental procedure for the transplantation of SA-BG-loaded hAECs/CM into mice with chemotherapy-induced POF