Table 4. Binding Constant Values Obtained for the Interaction of Ligand L₁ and Complex C₁ with the Calf Thymus DNA that was Followed by Fluorescence Spectroscopy.

		Kapp				K* = Kapp × nb
compound	expt	from double-reciprocal plot (a)	from non-linear plot (b)	from double-reciprocal plot with y-intercept = 1(c)	site size nb	from double-reciprocal plot (a)	from non-linear plot (b)	from double-reciprocal plot with y-intercept = 1(c)	K* from Scatchard	nb from Scatchard as nb = (n–1)
L1	1	2.80 × 103	3.30 × 103	1.60 × 103	8	2.20 × 104	2.60 × 104	1.28 × 104	1.86 × 104	8
	2	2.20 × 103	2.80 × 103	2.20 × 103	9	1.98 × 104	2.50 × 104	1.98 × 104	3.60 × 104	7
C1	1	0.94 × 104	0.80 × 104	0.70 × 104	13	1.20 × 104	1.04 × 104	0.91 × 104	1.53 × 104	15
	2	1.60 × 103	1.04 × 103	0.78 × 103	14	2.20 × 104	1.40 × 104	1.09 × 104	2.15 × 104	14