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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Mol Microbiol. 2020 Aug 12;114(5):823–838. doi: 10.1111/mmi.14582

Table 3.

Interactions between proteins from the N. gonorrhoeae T4SS.

Transmembrane/cytoplasmic proteins Periplasmic/outer membrane proteins cloned in both the BACHT and the BACHT-TM system Periplasmic proteins cloned only in the BACHT-TM system
T18TraCN TraCNT18 T18TraBN
TT18
T18TraEN TraGNT18 TraLNT18 T18AtlAN TM18AtlAN AtlANTT18 T18TraHN TM18TraHN T18TraKN TM18TraKN T18TraNN TM18TraNN T18TraUN TM18TraUN T18TraVN TM18TraVN TM18DsbCN TM18LtgXN TM18TraFN TM18TraWN TM18TrbCN TM18YagN

Transmembrane/cytoplasmic proteins T25TraCN +
TraCNT25 +
T25TraBN + + + + + + + w
T25TraEN
TraGN T25
TraLNT25

Periplasmic/outer membrane proteins cloned in both the BACHT and the BACHT-TM system T25AtlAN
TM25AtlAN
AtlANT25
T25TraHN
TM25TraHN + + +
T25TraKN +
TM25TraKN
T25TraNN
TM25TraNN
T25TraUN
TM25TraUN + +
T25TraVN +
TM25TraVN + +

Periplasmic proteins cloned only in the BACHT-TM system TM25DsbCN +
TM25LtgXN
TM25TraFN + +
TM25TraWN + +
TM25TrbCN +
TM25YagN

+, −, and w indicate respectively interaction, no interaction, and weak interaction. Space indicates that the interaction was not tested. The placement of T18 and T25 relative to the protein name indicates N or C-terminal fusion. T18 or T25 indicate that the gene encoding the protein was cloned into the BACTH vectors. TM18 or TM25 indicates that the gene encoding the protein was cloned into the BACTH-TM vectors.