Table 5.
Mixed interactions N. gonorrhoeae T4SS / F-plasmid T4SS (periplasmic and membrane-spanning proteins).
| Proteins from the F-plasmid T4SS | Proteins from the N. gonorrhoeae T4SS | |||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| T18TraBF | T18TraCF | TraCFT18 | T18TraEF | TM18TraFF | TM18TraHF | T18TraKF | TM18TraKF | TM18TraUF | T18TraVF | TM18TraVF | TM18TraWF | TM18TrbCF | TM18DsbCN | T18TraBN | T18TraCN | TraCNT18 | T18TraEN | TM18TraFN | TM18TraHN | T18TraKN | TM18TraKN | TM18TraUN | T18TraVN | TM18TraVN | TM18TraWN | TM18TrbCN | ||
| Proteins from the F-plasmid T4SS | T25TraBF | + | + | − | − | + | + | + | + | + | + | + | ||||||||||||||||
| T25TraCF | − | + | + | |||||||||||||||||||||||||
| TraCFT25 | − | − | − | |||||||||||||||||||||||||
| T25TraEF | − | |||||||||||||||||||||||||||
| TM25TraFF | − | − | − | + | − | |||||||||||||||||||||||
| TM25TraHF | − | − | − | − | − | − | ||||||||||||||||||||||
| T25TraKF | − | |||||||||||||||||||||||||||
| TM25TraKF | + | + | + | + | + | |||||||||||||||||||||||
| TM25TraUF | − | − | − | − | − | |||||||||||||||||||||||
| T25TraVF | − | |||||||||||||||||||||||||||
| TM25TraVF | − | − | − | − | − | − | − | − | − | |||||||||||||||||||
| TM25TraWF | − | − | − | + | − | |||||||||||||||||||||||
| TM25TrbCF | − | + | + | + | + | + | + | |||||||||||||||||||||
| Proteins from the N. gonorrhoeae T4SS | TM25DsbCN | − | + | |||||||||||||||||||||||||
| T25TraBN | + | + | + | − | + | − | + | + | + | + | ||||||||||||||||||
| T25TraCN | − | − | ||||||||||||||||||||||||||
| TraCNT25 | − | − | ||||||||||||||||||||||||||
| T25TraEN | − | |||||||||||||||||||||||||||
| TM25TraFN | + | − | − | − | − | |||||||||||||||||||||||
| TM25TraHN | + | − | + | + | − | |||||||||||||||||||||||
| T25TraKN | − | |||||||||||||||||||||||||||
| TM25TraKN | − | − | − | + | + | |||||||||||||||||||||||
| TM25TraUN | − | − | − | − | + | |||||||||||||||||||||||
| T25TraVN | − | |||||||||||||||||||||||||||
| TM25TraVN | + | − | − | − | − | + | − | + | ||||||||||||||||||||
| TM25TraWN | + | − | + | + | + | |||||||||||||||||||||||
| TM25TrbCN | − | − | − | − | − | − | + | |||||||||||||||||||||
+ and − indicate respectively interaction and no interactions. Space indicates that the interaction was not tested. The placement of T18 and T25 relative to the protein name indicates N or C-terminal fusion. T18 or T25 indicate that the gene encoding the protein was cloned into the BACTH vectors. TM18 or TM25 indicates that the gene encoding the protein was cloned into the BACTH-TM vectors.