Pairs of 60IIA>traRNAi
masculinized female Drosophila melanogaster use primarily female aggressive behavioral patterns and exhibit male-like courtship during fights. Fights between pairs of 60IIA>traRNAi masculinized females, pairs of 60IIA control females or pairs of traRNAi control females. Behavioral patterns were scored for 10 min from the first encounter (meeting between the flies). (A) Average number of head butts performed by females of the indicated genotypes. Each dot represents the average for one fight (Kruskal–Wallis test with Dunn's multiple comparison test). (B) Average number of lunge-like behavioral patterns displayed (Kruskal–Wallis test with Dunn's multiple comparison test). (C) Average number of lunges displayed (Kruskal–Wallis test with Dunn's multiple comparison test). (D) Average number of wing extensions performed (Kruskal–Wallis test with Dunn's multiple comparison test). (E) Percent of pairs that began fighting (showing head butts) over a 1-h period. (F) Percent of flies that performed a wing extension before a head butt (wing extension first), a head butt before a wing extension (head butt first), or that showed only head butts and no wing extensions (head butt only). For all histograms, *P<0.05, **P<0.01, ***P<0.001, n.s., not significantly different.