Canton-S males respond to masculinized females with male-like aggression. Fights between a Canton-S male and a female of the indicated genotype. (A,B) Percentage of fights in which the male lunged at the female (Fisher's two-tailed test). (C) Percentage of fights in which the male lunged at the elav>traRNAi female when the masculinized female was observed lunging or not lunging (Fisher's two-tailed test). (D) Percentage of fights in which the aggressive encounter escalated to boxing (Fisher's two-tailed test). (E) Percentage of fights in which the male lunged at the female when copulation was observed and when copulation was not observed (Fisher's two-tailed test). (F) Percentage of females copulating with a Canton-S male (chi-squared test). For all histograms, **P<0.01, ***P<0.001, n.s., not significantly different.