60IIA>traRNAi masculinized females exhibit sleep and activity patterns similar to those seen in wild-type females. Activity and sleep profiles of experimental and control flies. (A) Average activity profiles of wild-type males and females for days 3–5 of a 12 h:12 h light:dark cycle (LD). Zeitgeber time 0 (ZT0) indicates lights-on, ZT12 indicates lights-off. Dark gray bars indicate night. (B) Sleep patterns of Canton-S males and females during LD 3–5. (C) Total activity for days 3–5 of a 12 h:12 h LD cycle, measured by total number of beam crossings. (D) Average total daily sleep for days 3–5 during a LD cycle. (E) Total activity during the day phase (ZT0–ZT12), LD 3–5. (F) Total amount of sleep during the day phase (ZT0–ZT11). (G) Total activity during the night phase (ZT12–ZT23), LD 3–5. (H) Total amount of sleep during the night phase (ZT12–ZT23). For all histograms, *P<0.05, **P<0.01, ***P<0.001, n.s., not significantly different. For all activity plots, error bars represent means±s.e.m.