Fig. 4.

MINM does not require class V myosins Myo51 and Myo52. (A–E) Time points and kymographs of nuclear movement from movies of cells with the indicated genotypes and treatments. Di shows examples of normal nuclear movement in MBC-treated myo52Δ cells (48% of cells), while Dii shows examples of aberrant nuclear movement, including reversal of direction, in the same population (52% of cells). In E, ‘myoVΔ’ indicates double-mutant myo51Δ myo52Δ. Because essentially all DMSO-treated myoVΔ cells showed premature bipolar growth (see Materials and Methods), no DMSO-treated myoVΔ cells are shown. Time points (min) correspond to beginning and end of kymographs. Two examples are shown for each condition. Scale bar: 5 µm.