Fig. 6.

Zfp800 knockout mice are postnatally lethal. (A) Schematic of the Zfp800 gene and the location and sequence of the CRISPR/Cas9-generated 16 bp deletion. The guide crRNA sequence that caused the mutation is underlined and the PAM sequence is in a blue font. The deletion of 16 bp leads to a frameshift that abrogates normal ZFP800 662-aa protein translation and leads to translation of a truncated 41-aa peptide with 31 missense amino acids. (B) Representative image of a PCR genotyping gel for Zfp800 wild type (WT), heterozygous (Het) and knockout (KO). (C) RT-qPCR analysis of Zfp800 mRNA expression in pancreatic RNA samples collected at E18.5. n=3, error bars represent s.e.m.; ****P≤0.0001 (one-way ANOVA). (D) χ² analysis of progenies from Zfp800 Het×Het crossings genotyped at weaning (P21). No Zfp800 knockout animals survived past weaning demonstrating postnatal lethality with complete penetrance. (E) Zfp800 Het and WT animals have similar weights at different ages. n=8 (4 males and 4 females) for each genotype.