Fig. 4.

Identification of sex differences in wild-type E14.5 mice. (A,B) Plots showing WEV normalised organ volume against WEV for ovary (A) and lens (B). (C,D) A series of statistical tests was carried out with various combinations of male and female wild-type sample size (with females relabelled as ‘mutant’). Each test was repeated 50 times with randomly selected specimens. The values reported are the number of times the gonad volume was reported as significantly different (P<organ p threshold) in each set of tests for gonad (C) and lens of the eye (D). (E,F) Example results of Jacobian determinant analysis using eight females and eight males (E), and eight females and 46 males (F). Scale bars: 1 mm. RG, right gonad; LG, left gonad; A, anterior; P, posterior; L, left; R, right.