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. 2021 Mar 24;148(18):dev192955. doi: 10.1242/dev.192955

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Automated identification of pulmonary system phenotypes in a Wfdc2 knockout mouse line. (A) Organ volume plots of statistically significant organs. (B) Jacobian determinant analysis t-statistics (FDR corrected to q<0.1) overlaid on the E14.5 population average. Blue regions indicate smaller bronchi in the mutants. (C,D) Rigidly aligned sections of Wfdc2 mutants (bottom) and whole-embryo volume-matched wild-type rigidly aligned specimens (top), with arrows indicating the location of affected organs. (C) Coronal sections highlighting the bronchi. (D) Sagittal sections highlighting the trachea. Scale bars: 1 mm. A, anterior; P, posterior; S, superior; I, inferior; L, left; R, right; Tr, trachea; Br, bronchus.