Table 3.

Meloidogyne incognita egg hatch and second-stage juvenile (J2) activity in methanolic extracts from roots and shoots of Festulolium lines FHCD0802 BUS 10-12 U6 E+ and FHCD0802 U6 E−.

	Day 5	Day 7
	% active	% active
% DMSO in controls or µg/ml extract	Shootsa	Roots	Shoots
Water	97.1 a	94.3 a	94.3 a
Water + Kb	94.4 abc	94.5 a	94.5 a
0.5% DMSO	95.5 ab	93.7 ab	93.7 a
1.0% DMSO	94.7 ab	95.5 a	95.5 a
U6 E+ 200 µg/ml	87.8 cd	84.9 bc	85.3 b
	(8.1%)c	–	(9.0%)
U6 E− 200 µg/ml	89.7 d	81.8 c	83.6 b
	(6.1%)	(12.7%)	(10.8%)
U6 E+ 400 µg/ml	90.8 bcd	83.3 c	79.1 b
	–	(12.8%)	(17.2%)
U6 E− 400 µg/ml	88.8 cd	78.0 c	83.6 b
	(6.2%)	(18.3%)	(12.5%)

Notes: Eggs were immersed in the extracts. aFor day 5, means within a column followed by the same letter are not significantly different according to a Kruskal–Wallis test with a Wilcoxon test for each pair of multiple comparisons (P ≤ 0.05). For day 7, means within a column followed by the same letter are not significantly different according to Tukey’s adjustment for multiple comparisons (P ≤ 0.05); bWater + K = water plus kanamycin monosulfate, which was added to all treatments except the water control;

cNumbers in parentheses are percentage decreases in treatments that significantly reduced % active J2 compared with the corresponding controls: the 0.5% DMSO control for 200 ug/ml, and the 1.0% DMSO control for 400 ug/ml.