Table 5.
Detection of different virus concentrations by the eazyplex® SARS-CoV-2 using the INSTAND reference standard.
| Copies/mL | eazyplex® |
NeuMoDx™ |
||||
|---|---|---|---|---|---|---|
| Copies/reaction | Positive replicates, median threshold time, min (IQR) |
Positive replicates, N or/and ORF8-positive |
Positive replicates, median Ct |
|||
| N gene | ORF8 gene | N gene | Nsp2 gene | |||
| 106 | 240 | 6/6, 13 (12.75−13.75) | 6/6, 15.5 (13.75−20.25) | 6/6 | 4/4, 22.2 | 4/4, 23 |
| 105 | 24 | 4/6, 19.5 (16.5−20.25) | 4/6, 24.5 (23.5−24.75) | 6/6 | 4/4, 25.7 | 4/4, 25.9 |
| 104 | 3 | 1/6, 24.5 | 0/6 | 1/6 | 4/4, 29.1 | 4/4, 29.8 |
IQR, interquartile range.