REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
SARS-CoV-2/Hu/DP/Kng/19-020	Kanagawa Prefectural Institute of Public Health	N/A
Escherichia coli DH5-α	New England Biolabs	Cat#C2987
Chemicals, peptides, and recombinant proteins
100 unit/ml penicillin and 100 μg/ml streptomycin (P/S)	Sigma	Cat#09367-34
G418	Nacalai Tesque	Cat#09380-44
Blasticidin solution	InvivoGen	Cat#ant-bl
Doxycycline hydrochloride	Sigma	Cat#D3447-500MG
Trans IT LT-1	Mirus	Cat#MIR2306
PureLink RNA Mini Kit	Thermo Fisher Scientific	Cat#12183025
PrimeScript RT reagent kit (Perfect Real Time)	TaKaRa Bio	Cat#RR037A-3
PrimeSTAR GXL DNA polymerase	TaKaRa Bio	Cat#R050A
5′RACE System for Rapid Amplification of cDNA Ends, Version 2.0	Thermo Fisher Scientific	Cat#18374-041
DIG RNA Labeling kit (SP6/T7)	Roche	Cat#1175025910
DIG wash and block buffer set	Roche	Cat#11585762001
CDP-Star Chemiluminescent substrate	Sigma	Cat#c0721-100ml
Nano-Glo HiBiT Lytic detection system	Promega	Cat#N3030
Experimental models: Cell lines
Vero E6 cells	American Type Culture Collection	CRL-1586
BHK-21 cells	Japanese Collection of Research Bioresources Cell Bank	JCRB9020
TMPRSS2-expressing Vero E6 cells	Japanese Collection of Research Bioresources Cell Bank	JCRB1819
Tetracycline-inducible ACE2 and TMPRSS-expressing IFNAR1-deficient HEK293 (HEK293-3P6C33) cells	This paper	N/A
Oligonucleotides
CoV-2-G1-Fw (attaaaggtttataccttcccaggtaa caaaccaaccaactttcgatctc)	Fasmac	N/A
CoV-2-G1-Rv (tctacacaaactcttaa agaatgtataggg)	Fasmac	N/A
CoV-2-G2-Fw (cggcagtgaggacaatcagacaactac)	Fasmac	N/A
CoV-2-G2-Rv (ggcttagcataattagctatagtatcccaagggac)	Fasmac	N/A
CoV-2-G3-Fw (gccacgtataaaccaaatacctggtgtatacg)	Fasmac	N/A
CoV-2-G3-Rv (ggtgcacagcgcagcttcttcaaaagtactaaagg)	Fasmac	N/A
CoV-2-G4-Fw (agtggtagatgggtacttaacaatgattattac)	Fasmac	N/A
CoV-2-G4-Rv (gtttaaaaacgattgtgcatcagctgactg)	Fasmac	N/A
CoV-2-G5-Fw (acaacctaaatagaggtatggtacttggtag)	Fasmac	N/A
CoV-2-G5-Rv (gtggtttatgtgatttacaataatagctc)	Fasmac	N/A
CoV-2-G6-Fw (tgtagatgatatcgtaaaaacagatggtacac)	Fasmac	N/A
CoV-2-G6-Rv (aacgtgttatacacgtagcagactttagtggtac)	Fasmac	N/A
CoV-2-G7-Fw (aaaggttcaacacatggttgttaaagctgc)	Fasmac	N/A
CoV-2-G7-Rv (cgtacactttgtttctgagagagggtc)	Fasmac	N/A
CoV-2-G8-Fw (atgtttgtttttcttgttttattgccactag)	Fasmac	N/A
CoV-2-G8-Rv (aagttcgtttatgtgtaatgtaatttgactcc)	Fasmac	N/A
CoV-2-G9-Fw (gaaccacaaatcattactacagacaacac)	Fasmac	N/A
CoV-2-G9-Rv (gttcgtttaggcgtgacaagtttcattatg)	Fasmac	N/A
CoV-2-G10-Fw (atgtttcatctcgttgactttcaggttactatag)	Fasmac	N/A
CoV-2-G10-Rv (tttttttttttttttttttttttttgtcattctcc)	Fasmac	N/A
CoV-2-Race1 (gctgatgatcggctgcaacacggacg)	Fasmac	N/A
CoV-2-Race2 (gccacacagattttaaagttcgtttagag)	Fasmac	N/A
CoV-2-F1-Fw (CTATATAAGCAGAGCTCGTTTAGTGA ACCGTattaaaggtttataccttcccaggtaac)	Fasmac	N/A
CoV-2-F1-Rv (cagattcaacttgcatggcattgttagtagccttatttaaggctcctgc)	Fasmac	N/A
CoV-2-F2-Fw (gcaggagccttaaataaggctactaacaatgccatgcaagttgaatctg)	Fasmac	N/A
CoV-2-F2-Rv (ggtaggattttccactacttcttcagagactggttttagatcttcgcaggc)	Fasmac	N/A
CoV-2-F3-Fw (gcctgcgaagatctaaaaccagtctctgaagaagtagtggaaaatcctacc)	Fasmac	N/A
CoV-2-F3-Rv (ggtgcacagcgcagcttcttcaaaagtactaaagg)	Fasmac	N/A
CoV-2-F4-Fw (caccactaattcaacctattggtgctttggacatatcagcatctatagtagctggtgg)	Fasmac	N/A
CoV-2-F4-Rv (gtttaaaaacgattgtgcatcagctgactg)	Fasmac	N/A
CoV-2-F5-Fw (cacagtctgtaccgtctgcggtatgtggaaaggttatggctgtagttgtgatc)	Fasmac	N/A
CoV-2-F5-Rv (gcggtgtgtacatagcctcataaaactcaggttcccaataccttgaagtg)	Fasmac	N/A
CoV-2-F6-Fw (cacttcaaggtattgggaacctgagttttatgaggctatgtacacaccgc)	Fasmac	N/A
CoV-2-F6-Rv (catacaaactgccaccatcacaaccaggcaagttaaggttagatagcactctag)	Fasmac	N/A
CoV-2-F7-Fw (ctagagtgctatctaaccttaacttgcctggttgtgatggtggcagtttgtatg)	Fasmac	N/A
CoV-2-F7-Rv (ctagagactagtggcaataaaacaagaaaaacaaacattgttcgtttagttgttaac)	Fasmac	N/A
CoV-2-F8-Fw (gttaacaactaaacgaacaatgtttgtttttcttgttttattgccactagtctctag)	Fasmac	N/A
CoV-2-F8-Rv (gcagcaggatccacaagaacaacagcccttgagacaactacagcaactgg)	Fasmac	N/A
CoV-2-F9-Fw (ccagttgctgtagttgtctcaagggctgttgttcttgtggatcctgctgc)	Fasmac	N/A
CoV-2-F9-Rv (caatctccattggttgctcttcatc)	Fasmac	N/A
CoV-2-F10-Fw (gatgaagagcaaccaatggagattg)	Fasmac	N/A
CoV-2-F10-Rv (GGAGATGCCATGCCGACC Ctttttttttttttttttttttttttgtcattctcctaag)	Fasmac	N/A
CoV-2-Linker-Fw (cttaggagaatgacaaaaaaaaaaaa aaaaaaaaaaaaaGGGTCGGCATGGCATCTCC)	Fasmac	N/A
CoV-2-Linker-Rv (gttacctgggaaggtataaacctttaatA CGGTTCACTAAACGAGCTCTGCTTATATAG)	Fasmac	N/A
ORF6-HiBiT-Rv (ccGCTAATCTTCTTGAACAGCCGCCAGCCGCTCA Ccatctgttgtcacttactgtacaagcaaagcaatattgtcactgc)	Fasmac	N/A
ORF6-HiBiT-Fw (CTGTTCAAGAAGATTAGCgggagttctggctttcatct cgttgactttcaggttactatagcagagatattactaattat)	Fasmac	N/A
Recombinant DNA
pCSII-CoV-2-G1	This paper	N/A
pCSII-CoV-2-G2	This paper	N/A
pCSII-CoV-2-G3	This paper	N/A
pCSII-CoV-2-G4	This paper	N/A
pMW-CoV-2-G5	This paper	N/A
pMW-CoV-2-G6	This paper	N/A
pCSII-CoV-2-G7	This paper	N/A
pCSII-CoV-2-G8	This paper	N/A
pCSII-CoV-2-G9	This paper	N/A
pCSII-CoV-2-G10	This paper	N/A
pMW-CoV-2-UTRlinker	This paper	N/A
pCSII-CoV-2-G10-sfGFP	This paper	N/A
pCAGGS-CoV-2-N	This paper	N/A