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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Immunol Rev. 2020 Apr 1;295(1):101–113. doi: 10.1111/imr.12853

Figure 1. Obese adipose tissue macrophages (ATMs) display a hypermetabolic phenotype, different from M1 or M2 polarized bone marrow derived macrophages (BMDMs).

Figure 1.

A-B) BMDMs from C57BL/6J mice were polarized for 24 hr with 10 ng/mL LPS/IFNy (M1) or IL-4/IL1-3 (M2). A) A mitochondrial stress test was completed on a Seahorse metabolic flux analyser for basal and maximal oxygen consumption rate (OCR) and B) basal extracellular acidication rate (ECAR). C-D) ATMs were isolated from C57BL/6J mice on a low fat diet (LFD) or high fat diet (HFD) for 9 wks and C) OCR and D) ECAR were assessed.

*p<0.05, **p<0.01, ***p<0.001, ***p<0.0001 by two-way ANOVA (A and C), one way ANOVA (B), or t-test (C and D) and post-hoc testing where appropriate.