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. Author manuscript; available in PMC: 2021 Apr 26.
Published in final edited form as: Nat Metab. 2020 Oct 26;2(11):1248–1264. doi: 10.1038/s42255-020-00288-1

Extended Data Fig. 2. Glycolysis enzymes and intermediates in cMPC1−/− hearts.

Extended Data Fig. 2

(a) Glycolysis-derived metabolic intermediates in 8-week-old control and cMPC1−/− hearts were determined by GC-MS. Mice were random fed before sacrifice. (Control, 15; cMPC1−/−, 11). (b) hexokinase I (HK I), GAPDH, O-GlcNAc transferase (OGT) and glycogen synthase (GS) blots were performed in lysates of cardiomyocytes isolated from 8-week-old control and cMPC1−/− mice. Protein quantification was normalized to total Coomassie blue staining. (n=5 both groups). Data are presented as mean ± SEM and analyzed by two-tailed unpaired Student’s t-test.