Skip to main content
. Author manuscript; available in PMC: 2021 Apr 26.
Published in final edited form as: Nat Metab. 2020 Oct 26;2(11):1248–1264. doi: 10.1038/s42255-020-00288-1

Figure 8. Divergent effects of ketogenic diet feeding on pressure overload-induced cardiac remodeling in cMPC1−/− mice.

Figure 8.

Control and cMPC1−/− mice were subjected to TAC surgery at the age of 6 weeks and heart weights or lung weights normalized to tibia length (a-b) and ejection fraction (c) were determined 3 weeks post TAC. Cardiac fibrosis was revealed by Trichrome staining (d-e) at time of sacrifice. Scale bar, 200 μm. Images are representative of n= 3 per group. Transverse aortic constriction surgery was performed on 8-week-old control and cMPC1−/− mice that were maintained on chow diet or initiated on a ketogenic diet (KD) at the time of TAC surgery. The survival curve following TAC is shown in panel f. Control and cMPC1−/− mice were fed with ketogenic diet at weaning and were subjected to TAC surgery at the age of 6 weeks. Ejection fraction (g) and heart weights or lung weights normalized to tibia length (h, i) were assessed at 3 weeks post TAC. For panels a-c and e, n=3 (all groups); for panel F, n=6 (Control-2920X), n=6 (Control-Keto), n=7 (cMPC1−/−−2920X), n=8 (cMPC1−/−-Keto); for panel g-i, n=5 (Control-2920X), n=5 (cMPC1−/−−2920X), n=7 (Control-Keto), n=6 (cMPC1−/−-Keto). Data are presented as mean ± SEM and P value was determined by two-way ANOVA followed by Tukey multiple comparison test.