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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Nat Chem Biol. 2021 Mar 1;17(4):383–386. doi: 10.1038/s41589-021-00748-z

Figure 1: The corrected GPR37L1 construct does not have the apparent Gαs constitutive activity seen with p426-r37L1.

Figure 1:

(a–b) Examination of the effects of p426-r37L1 on reporter signal in (a) CRE-luc and (b) CAMYEL assays. HEK293 cells were transiently co-transfected with the CRE-luc reporter or the CAMYEL BRET biosensor together with incorrect or corrected GPR37L1 construct or β2-adrenoceptor, as indicated. ‘Empty’ refers to empty plasmid DNA. For CRE-luc assay, data represent n=2 biological replicates each performed in triplicate on separate days; each data point is the average of the technical replicates for the corresponding biological replicate, and the bar height indicates the mean of biological replicates. For CAMYEL assay, data represent n=1 with technical replicates shown as dots. RLU, relative light units; FSK, forskolin. (c) Western blot for transient HEK293 cellular expression of r37L1, corrected GPR37L1 or controls, as indicated. Cerebellum from a male C57BL/6J mouse was used as a positive control. Dashed lane contained protein ladder only. Image is n=1. All raw data is available in the accompanying Source Data file.