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Sequence tagging of NORs and transfer to human cells. (A) Organization of NORs on acrocentric p-arms, depicting rDNA arrays and proximal and distal junctions (PJs and DJs). A representation of a sequenced tagged NOR is shown below. (B) MMCT workflow for transfer of human chromosomes with edited NORs from A9 hybrid donor cells into recipient hTERT-RPE1 Hyg−ve cells.
