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. 2021 Apr 1;35(7-8):483–488. doi: 10.1101/gad.348234.121

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© 2021 Mangan and McStay; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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Figure 3. — Genome-edited rDNA arrays yield functional ribosome subunits. (A) Hybrids used in genome editing, sequence tags, and post-MMCT cell lines. (B) Northerns of total RNA and RNA isolated from ribosome and polysome fractions of RPE1, 14/3(28S), and 15/3(ETS/28S) cells, using a 28S-tag DIG-labeled probe. (C) RT-PCR reveals the proportion of 28SrRNA deriving from edited NORs in 14/3(28S) and 15/3(28SMS2) ribosome and polysome fractions. (D) RNA-FISH identifies cytoplasmic tagged 28SrRNA in 14/3(28S) cells. Nucleoli are visualized with an 18SE oligo probe. Scale bar, 5 µm.