Figure 1.

Human interacting partners of DENV-NS5 curated from various experimental studies and databases. (A) The Venn diagram indicates the number of DENV-NS5 interacting proteins that are shared with and/or unique to PPI studies in the literature viz., yeast-two-hybrid studies, pull-down studies, and bioinformatics. Yeast-two-hybrid data were curated from the DenvInt database, bioinformatics-based data was obtained from P-HIPSTer, and pull-down data has been derived from published data sources. All cited sources and extended data are compiled and listed in Supplementary Table 1 . (B) Some of the NS5 interactors involvement in key KEGG pathways as obtained using the WEB-based GEne SeT AnaLysis Toolkit. The interactors are grouped in boxes based on the key pathways that they are involved in as obtained from KEGG. The proteins are color-coded according to the method used for their identification. A list of all significant pathways with a false discovery rate (FDR) < 0.05 is given in Supplementary Table 2 . The complete results of the GO filtering are shown in Supplementary Figure 1 . SPTAN1 is the only protein detected by both bioinformatics and yeast two-hybrid experiments but has not been shown here because it was associated with a false discovery rate > 0.05 which is the threshold for our compilation.