Table 1.
Prime sequences for quantitative reverse transcription followed by polymerase chain reaction (qRT-PCR).
| Gene | Primer Sequences (5′-3′) | |
|---|---|---|
| β-actin | ||
| sense | CCAGCCTTCCTTCTTGGGTAT | |
| antisense | TTGGCATAGAGGTCTTTACGG | |
| Kcnd2 | ||
| sense | TGACAACACTGGGGTATGGC | |
| antisense | CCGACTGAAGTTCGACACGA | |
| Kcnj2 | ||
| sense | TCTCACTTGCTTCGGCTCAT | |
| antisense | ACTTGTCCTGTTGCTGGTACA | |
| Irx5 | ||
| sense | GCCTTCTCTTACGTGGGCTC | |
| antisense | AGTGGCATTCTTCCGGTACG | |
| α1c (Cacna1c) | ||
| sense | TCCCGAGCACATCCCTACTC | |
| antisense | ACTGACGGTAGAGATGGTTGC | |
| Bax | ||
| sense | AAACTGGTGCTCAAGGCCC | |
| antisense | CTTGGATCCAGACAAGCAGC | |
| Bcl-2 | ||
| sense | GCTACCGTCGTGACTTCGC | |
| antisense | CCCACCGAACTCAAAGAAGG | |
| Casp9 | ||
| sense | TCAGGGGACATGCAGATATGG | |
| antisense | TTGGCAGTCAGGTCGTTCTTC | |
| U6 | ||
| sense | ATGACGTCTGCCTTGGAGAAC | |
| antisense | TCAGTGTGCTACGGAGTTCAG | |
| Mmu-miR-1a-3p | ||
| reverse | transcription | prime |
| CTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGAGATACAT | ||
| sense | GCCGCTGGAATGTAAAGAAGT | |
| antisense | GTCCGAGGTATTCGCACTGGATA | |
| Mmu-miR-133a-3p | ||
| reverse | transcription | prime |
| GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAGCTG | ||
| sense | GCTTTGGTCCCCTTCAAC | |
| antisense | GTCCGAGGTATTCGCACTGGATA | |