Table 2. Thermodynamic Parameters Evaluated from Micro-DSC Investigations for Several FFA-Containing Vesicles Alone and in the Presence of 30 μM Nisin^a.

	calorimetry
	ΔH° (kJ·mol–1)	Tmax (°C)	T̅ (°C)	ACI (°C)	spectroscopy Tflex (°C)
model membrane (REF)	26 ± 2	32.6 ± 0.6	30.1 ± 0.1	5.3 ± 0.1	30.8 ± 0.4
REF + nisin	28 ± 2	34.3 ± 0.5	30.7 ± 0.1	5.6 ± 0.1	–
REF + PA	30 ± 2	39.5 ± 0.4	34.9 ± 0.2	7.3 ± 0.2	37.1 ± 0.6
REF + PA + nisin	35 ± 2	39.3 ± 0.7	34.4 ± 0.2	7.1 ± 0.2	–
REF + SA	30 ± 2	39.9 ± 0.6	36.6 ± 0.2	7.6 ± 0.2	38.1 ± 0.7
REF + SA + nisin	28 ± 2	41.5 ± 0.2	36.6 ± 0.2	7.4 ± 0.2	–
REF + EA	30 ± 2	35.0 ± 0.6	31.9 ± 0.2	5.5 ± 0.2	33.8 ± 0.7
REF + EA + nisin	28 ± 2	36.0 ± 0.2	32.7 ± 0.2	5.7 ± 0.2	–
REF + OA	24 ± 2	30.6 ± 0.6	28.1 ± 0.2	5.1 ± 0.2	28.8 ± 0.6
REF + OA + nisin	27 ± 2	29.6 ± 0.5	29.1 ± 0.2	5.7 ± 0.2	–
REF + LA	25 ± 2	29.9 ± 0.4	28.5 ± 0.2	6.1 ± 0.2	27.7 ± 0.5
REF + LA + nisin	20 ± 2	26.8 ± 0.5	25.8 ± 0.2	5.7 ± 0.2	–
REF + DHA	18 ± 2	28.3 ± 0.9	27.6 ± 0.2	5.9 ± 0.2	25.9 ± 1.5
REF + DHA + nisin	29 ± 2	32.0 ± 0.5	29.3 ± 0.2	5.9 ± 0.2	–

^aThe last cycle heating curves were used to obtain the main transition enthalpy (ΔH°), the peak maximum temperature (T_max), the transition average temperature (T̅), and the average cooperativity index (ACI). The transition average temperature (T̅) was also compared to the temperature at the sigmoid flex point (T_flex) obtained from fluorescence spectroscopy measurements. The abbreviations in the table indicate palmitic acid (PA), stearic acid (SA), elaidic acid (EA), oleic acid (OA), linoleic acid (LA), and docosahexaenoic acid (DHA).