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. 2021 Mar 22;15(3):e0009303. doi: 10.1371/journal.pntd.0009303

Fig 3. In situ identification of proliferative cells in Taenia solium cyst samples by ISH.

Fig 3

Plk1-expressing cells were observed in racemose larvae (A) and were limited to the neck of scolex in vesicular larvae (C) (arrowheads). Proliferative cell were absent in the bladder wall of vesicular larvae samples (B). A higher magnification image is inserted (A,C). A sense probe of plk1 (D) was used as a negative control and antisense probe for actin as a positive control (E) in vesicular larvae where strong staining of muscle fibers was observed (arrows). Differential expression of plk1 gene in racemose form (F). Quantitative PCR of cDNA from 500 ng of total RNA from racemose form compared to the scolex and bladder wall of vesicular form. Results are normalized to the housekeeping gapdh gene and expressed as fold increase in plk1 expression. Statistically significant differences in levels of gene expression are indicated by asterisks (Mann-Whitney U test). Asterisks represent level of significance: *: p<0.05; **: p<0.01. BW: Bladder wall; S: Scolex.