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. 2021 Mar 22;15(3):e0009303. doi: 10.1371/journal.pntd.0009303

Fig 8. Evaluation of supernatants by Ag-ELISA and RT-PCR of cell cultures.

Fig 8

Evaluation of supernatants obtained from proliferative cell cultures with different densities using anti-T. solium monoclonal antibodies (A). The values were expressed as a ratio by dividing the optical density (OD) of the samples by the OD of the cut-off value. An ELISA ratio >1 was considered as positive. Excretory/Secretory antigens were used as positive controls (1 and 2) and supplemented RPMI culture medium as the negative control. Agarose gel electrophoresis of RT-PCR (B). cDNA was generated from the total RNA of three cell cultures of racemose larvae. The specific amplification product (~266 bp) for β-actin gene was only observed in the positive control human blood cell cDNA (left arrow). M: 100 bp DNA ladder; 1–3: samples of proliferative cell cultures; 4: positive control; 5: master mix.