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. 2021 Mar 22;17(3):e1009267. doi: 10.1371/journal.pgen.1009267

Fig 5. Pol θ suppresses mitotic crossing over.

Fig 5

A) Third chromosomes (maternal, ♀, and paternal, ♂) of a male fly expressing Cas9 and a gRNA targeting the ry gene, and heterozygous for the markers st and e. The PAM sequence for the gRNA in the maternal chromosome, and lack thereof in the paternal one, is indicated. Wild-type ry (+) in the maternal chromosome indicates either unedited (if PAM is present) or HR repair (if PAM is absent). Mutant ry (-) indicates repair by mutagenic end joining. B) Percent of chromosomes repaired by HR, EJ, or unedited, calculated by assessing the ry and PAM status. n = 56. C) Third chromosomes of a male fly described in panel A before and after the generation of a mitotic crossover. D) Percent of total progeny that were recombinant is indicated for each genotype (like those described in panels A and C). Males were maternally and zygotically mutant for the indicated genes. Number of males crossed: 97 (wild type), 55 (POLQ), 62 (SLX4), 60 (GEN1), 69 (SLX4 GEN1). Number of progeny scored: 8790 (wild type), 3471 (POLQ), 5016 (SLX4), 6830 (GEN1), 3446 (SLX4, GEN1). Error bars represent 95% CI. Statistical significance was assessed by one-way ANOVA with Bonferroni correction to account for multiple comparisons; ns, not significant; *, p <0.05.