Table 3. Percent of tissues testing positive for RVFV via plaque assay among progeny mosquitoes.
| F1 E1 Mosquitoes | F1 E2 Mosquitoes | F1 E3 Mosquitoes | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Tested | Positive | Percent Infected† | Tested | Positive | Percent Infected† | Tested | Positive | Percent Infected† | |
| Egg Rafts | 5 | 0 | 0% | 5 | 4 | 80% | 5 | 0 | 0% |
| 1st Instar Larvae (pools of 5) | 30 | 2 | 1%* | 30 | 2 | 1%* | - | - | - |
| 2nd Instar Larvae (pools of 5) | 30 | 4 | 3%* | 30 | 0 | 0%* | - | - | - |
| 3rd Instar Larvae | 50 | 4 | 8% | 50 | 6 | 12% | - | - | - |
| 4th Instar Larvae | 50 | 3 | 6% | 50 | 0 | 0% | - | - | - |
| Pupae-Male | 50 | 4 | 8% | 50 | 1 | 2% | - | - | - |
| Body-Male | 50 | 1 | 2% | 50 | 6 | 12% | 50 | 0 | 0% |
| Pupae-Female | 50 | 0 | 0% | 50 | 2 | 4% | - | - | - |
| Body-Female | 50 | 1 | 2% | 50 | 5 | 10% | 50 | 5 | 10% |
| Legs & Wings-Female | 50 | 4 | 8% | 50 | 1 | 2% | 50 | 3 | 6% |
| Saliva-Female | 50 | 0 | 0% | 50 | 2 | 4% | 50 | 2 | 4% |
| Ovaries-Female | 50 | 2 | 4% | 50 | 3 | 6% | 50 | 7 | 14% |
†Percent infection rates for pooled samples were calculated using maximum likelihood estimation. These values are indicated with an asterisk.