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. 2021 Mar 22;17(3):e1009446. doi: 10.1371/journal.pgen.1009446

Fig 3. Defective NCCs differentiation in the pharyngeal arch arteries of neural crest-specific BAF155/170 knockout embryos.

Fig 3

(A-H) Lineage tracing of Wnt1Cre/+-derived NCCs using R26mTmG/+ reporter at E9.5 (A-D), E10.5 (E-H) and E11.5 (I-L). Control, BAF170-deficient (Wnt1Cre/+;BAF170fl/fl;BAF155fl/+), BAF155-deficient (Wnt1Cre/+;BAF155fl/fl;BAF170fl/+) and BAF155/170-deficient (Wnt1Cre/+;BAF155fl/fl;BAF170fl/fl) embryos were analyzed by GFP and RFP immunofluorescence (n = 4 each genotype). Merged GFP/RFP images are presented here (A-L). Scale bars 200μM (A-H) and 500μM (I-L). (M-P) Anti-GFP immunostaining on the frontal sections of E11.5 control (M), BAF170-deficient (N), BAF155-deficient (O), and BAF155/170-deficient (P) embryos showing neural crest-derived cells in the pharyngeal arches (n = 3 each genotype). Scale bar 75μM (M-P). (Q-T) Anti-SM22α immunostaining on the frontal sections of E11.5 control (Q), BAF170-deficient (R), BAF155-deficient (S), and BAF155/170-deficient (T) embryos showing differentiation of neural crest-derived cells into the smooth muscle of the 4th pharyngeal arch artery (n = 3 each genotype). Scale bar 50μM (Q-T). fl, forelimb; hl, hindlimb; ht, heart; mc, metencephalon; pa, pharyngeal arch; paa, pharyngeal arch artery; tc, telencephalon.