Fig 6. Gene expression changes in isolated NCCs due to BAF155/170 deletion.

(A) Experimental design for RNAseq analysis. Single-cell suspensions were prepared from lineage traced control (Wnt1^Cre/+:BAF155^flox/+:BAF170^flox/+:Rosa26^mTmG/+) and BAF155/170-deficient (Wnt1^Cre/+:BAF155^flox/flox:BAF170^flox/flox:Rosa26^mTmG/+) embryos for Fluorescence-activated cell sorting (FACS). NCCs that were positive for GFP were collected from control and BAF155/170-deficient embryos for RNA isolation and subsequently for library preparation and RNAseq analysis. (B) Heat map of differentially expressed transcripts (3011 genes) from RNAseq analysis of sorted control and BAF155/170-deficient NCCs. Genes regulating different aspects of neural crest biology including proliferation and differentiation were significantly down-regulated in BAF155/170-deficient NCCs compared to the controls. In contrast, genes regulating apoptosis were up-regulated in BAF155/170-deficient NCCs compared to controls. (n = 3 each genotype).