Fig 4. Differential expression analyses of all identified miRNAs between sample types representing different in vivo transitions or in vitro cultured conditions.

MiRNAs that were expressed in both stages but showed log2 fold change ≥1 and p-adjusted <0.05 are shown. PC1 vs PC2-3 (A). MCana vs PC2-3 (B). naPS vs MCvitro (C). MCvivo vs MCana (D). MCvivo: metacestodes extracted from experimentally infected jirds, MCvitro: metacestodes cultured in vitro in aerobic conditions, MCana: metacestodes cultured in vitro in axenic and anaerobic conditions, PC1: primary cell cultured in vitro for 48 hs, PC2-3: primary cells cultured in vitro for 7–21 days (see methods section), naPS: non-activated protoscoleces and aPS: activated protoscoleces.