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. 2021 Mar 22;15(3):e0009297. doi: 10.1371/journal.pntd.0009297

Fig 5. Whole-mount in situ hybridization (WMISH) of miR-71 in Echinococcus multilocularis metacestodes cultured in vitro (MCvitro).

Fig 5

Maximum intensity projection of (A) and (C) germinal layer of E. multilocularis metacestodes; (B) and (D) germinal layer containing brood capsules (BC) and developing protoscoleces (DP). Nuclear labeling with DAPI (A.1 and B.1, blue); WMISH signal of miR-71 (A.2, B.2, C.2 and D.2, green); detection of proliferation marker EdU (C.1, D.1, magenta); merge of nuclear labeling with DAPI and WMISH signal of miR-71 (A.3 and B.3); and merge of detection of proliferation marker EdU and WMISH signal of miR-71 (C.3 and D.3). Dotted lines delimit BC and DP structures. Scale bar of 10 μm in A, C; 50 μm in B; 20 μm in D.