Table 1. The repertoire of Echinococcus multilocularis mature miRNAs identified through metacestode development in vitro, their evolutionary origin and conservation.

miRNA name	Mature sequence (5’-3’)a	Family	Evolutionary originb	Conservation in selected Phylac	Conservation in selected Platyhelminthesd
					Egr	Eca	Tcr	Mvo	Hmi	Sma	Gsa	Sme
emu-bantam-3p	UGAGAUCGCGAUUACAGCUGAU	Bantam	P	+/+/+/–/–	+	+	+	+	+	+	+	+
emu-let-7-5p	UGAGGUAGUGUUUCGAAUGUCU	let-7	B	+/+/+/+/–	+	+	+	+	+	+	+	+
emu-miR-1-3p	UGGAAUGUUGUGAAGUAUGU	mir-1	B	+/+/+/+/–	+	+	+	+	+	+	+	+
emu-miR-2a-3p	AAUCACAGCCCUGCUUGGAACC	mir-2	P	+/+/+/–/–	+	+	+	+	+	+	+	+
emu-miR-2b-3p	UAUCACAGCCCUGCUUGGGACA				+	+	+	+	+	+	+	+
emu-miR-2c-3p	__UCACAGCCAAUAUUGAUGAA				+	+	+	+	+	+	+	+
emu-miR-7a-5p	UGGAAGACUGGUGAUAUGUUGU	mir-7	B	+/+/+/+/–	+	+	+	+	+	+	+	+
emu-miR-7b-5p	UGGAAGACUUGUGAUUAGAUUGUU				+	+	+	+	+	+	+	+
emu-miR-8-3p	UAAUACUGUUCGGUUAGGACGCC	mir-8	B	+/+/+/+/–	+	+	-	-	-	+	+	+
emu-miR-9-5p	UCUUUGGUUAUCUAGCUGUGUG	mir-9	B	+/+/+/+/–	+	+	+	+	+	-	+	+
emu-miR-10-5p	CACCCUGUAGACCCGAGUUUGA	mir-10	E	+/+/+/+/+	+	+	+	+	+	+	+	+
emu-miR-31-5p	UGGCAAGAUACUGGCGAAGCUGA	mir-31	B	+/+/+/+/–	+	+	+	+	+	+	+	+
emu-miR-36a-3p	UCACCGGGUAGACAUUCCUUGC	mir-36	P	+/+/+/–/–	+	+	+	+	+	+	+	+
emu-miR-36b-3p	UCACCGGGUAGUUAUUACGCCU				+	+	+	+	+	+	+	+
emu-miR-61-3p	UGACUAGAAAGAGCACUCACAUC	mir-61/279	P	+/+/+/–/–	+	+	+	+	+	+	+	+
emu-miR-71-5p	UGAAAGACGAUGGUAGUGAGA	mir-71	B	+/+/+/–/–	+	+	+	+	+	+	+	+
emu-miR-87-3p	GUGAGCAAAGUUUCAGGUGU	mir-87	P	+/+/+/–/–	+	+	+	+	+	-	+	+
emu-miR-96-5p	AUUGGCACUUUUGGAAUUGUC	mir-96	B	+/+/+/+/–	+	+	+	+	+	+	+	+
emu-miR-124a-3p	UAAGGCACGCGGUGAAUGCCA	mir-124	B	+/+/+/+/–	+	+	+	+	+	+	+	+
emu-miR-124b-3p	UAAGGCACGCGGUGAAUACC				+	+	+	+	+	+	+	+
emu-miR-125-5p	UCCCUGAGACCCUAGAGUUGUC	mir-125	B	+/+/+/+/–	+	+	+	+	+	+	+	+
emu-miR-133-3p	UUGGUCCCCAUUAACCAGCCGCC	mir-133	B	+/+/+/+/–	+	+	+	+	+	-	-	+
emu-miR-153-3p	UUGCAUAGUCUCAUAAGUGCCA	mir-153	B	+/+/+/+/–	+	+	+	+	+	-	+	+
emu-miR-184-3p	GGGACGGAAGUCUGAAAGGUUU	mir-184	B	+/+/+/+/–	+	+	+	+	+	-	+	+
emu-miR-190-5p	AGAUAUGUUUGGGUUACUUGGUGCU	mir-190	B	+/+/+/+/–	+	+	+	+	+	+	+	+
emu-miR-219-5p	UGAUUGUCCAUUCGCAUUUCUUG	mir-219	B	+/-/+/+/–	+	+	+	+	+	+	+	+
emu-miR-277a-3p	UAAAUGCAUUUUCUGGCCCGUA	mir-277/4989	P	+/+/+/–/–	+	+	+	+	+	+	+	+
emu-miR-277b-3pe	UAAAUGCAAAAUAUCUGGUUAUG				+	+	+	+	+	+	+	+
emu-miR-4989-3p	AAAAUGCACCAACUAUCUGAGA				+	+	+	+	+	+	+	+
emu-miR-281-3p	UGUCAUGGAGUUGCUCUCU	mir-281	B	+/+/+/+/–	+	+	+	+	+	+	+	+
emu-miR-307-3p	UCACAACCUACUUGAUUGAGGGG	mir-307/67	P	+/+/+/–/–	+	+	+	+	+	-	+	+
emu-miR-745-3p	UGCUGCCUGGUAAGAGCUGUGA	mir-745/22	B	+/–/+/+/–	+	+	+	+	+	+	-	+
emu-miR-1992-3p	UCAGCAGUUGUACCAUUGAAAU	mir-1992	L	+/–/–/–/–	+	+	+	-	-	-	+	+
emu-miR-2162-3p	UAUUAUGCAACUUUUCACUCC	mir-2162/1993	P	+/+/+/–/–	+	+	+	+	+	+	+	+
emu-miR-3479a-3p	UAUUGCACGUUCUUUCGCCAUC	mir-3479/92	B	+/+/+/+/–/	+	+	+	+	+	+	-	+
emu-miR-3479b-3p	GAUUGCACUACCCAUCGCCCAC				+	+	+	+	+	+	-	+
emu-miR-10293-3pf	UAAUUCGAGUCAACAGGGUCGUU	mir-10293	Pl	–/–/–/–/–	+	+	+	+	-	+	+	+

^a Seed sequences (nt 2–8) are shown in bold.

^b P: Protostomia; B: Bilateria, E: Eumetazoa; L: Lophotrochozoa; Pl: Platyhelminthes.

^c Annelida/Nematoda/Arthrophoda/Vertebrata/Cnidaria. (Vertebrata is a Subphylum).

^d Egr: Echinococcus granulosus, Eca: Echinococcus canadensis, Tcr: Taenia crassiceps, Mvo: Mesocestoides vogae, Hmi: Hymenolepis microstoma, Sma: Schistosoma mansoni, Gsa: Gyrodactylus salaris, Sme: Schmidtea mediterranea.

^e Previously reported as miR-new-2-3p by Cucher et al. (2015).

^f Previously reported as miR-new-1-3p by Cucher et al. (2015).