Figure 4. CIE-induced enhancement of DSE at OFC terminals to D1 SPNs in the DS.

(A) Schematic of viral injections in OFC and optical stimulation of OFC terminals during whole-cell recordings of D1 SPNs in the DS. (B) Depolarization-induced suppression of excitation (DSE) of OFC inputs to D1 SPNs in Air (n = 8 cells, three mice) and CIE (n = 9 cells, three mice). (C) Bar graphs representing the percentage change from baseline immediately after depolarization. (D) Schematic of local electrical stimulation during whole-cell recordings of D1 SPNs in the DS. (E) DSE of excitatory inputs to D1 SPNs using electrical stimulation in Air (n = 6 cells, two mice) and CIE (n = 6 cells, three mice). (F) Bar graphs representing the percentage change from baseline immediately after depolarization. Scale bars represent 10 ms (horizontal) and 50 pA (vertical). Data points and bar graphs represent mean ± SEM. Scale bars represent 10 ms (horizontal) and 50 pA (vertical). Unpaired (Air vs. CIE) or paired (vs. baseline) two-tailed t-test, **p≤0.01, ***p≤0.001, ****p≤0.0001.

Figure 4—figure supplement 1. DSE in D2 SPNs and effect of SR141716 on DSE in D1 SPNs.

(A) Depolarization-induced suppression of excitation (DSE) of OFC inputs to D2 SPNs in Air control and CIE mice. (B) Bar graphs representing the percentage change from baseline immediately after depolarization. (C) DSE of excitatory inputs to D2 SPNs using electrical stimulation in Air and CIE mice. (D) Bar graphs representing the percentage change from baseline immediately after depolarization. (E) DSE of OFC inputs to D1 SPNs in the presence of a CB1 receptor antagonist, SR141716 (1 µM) in Air and CIE mice. (F) Bar graphs representing the percentage change from baseline immediately after depolarization. (G) DSE of excitatory inputs to D1 SPNs using electrical stimulation in the presence of a CB1 receptor antagonist, SR141716 (1 µM) in Air and CIE mice. (H) Bar graphs representing the percentage change from baseline immediately after depolarization. Data points and bar graphs represent the mean ± SEM. Paired (vs. baseline) two-tailed t-test, **p≤0.01, ***p≤0.001.

Figure 4—figure supplement 2. CIE-induced effects on D1 receptor function and DSE across recording days.

(A) Experimental timeline that includes surgeries followed by four cycles of CIE exposure and whole-cell recordings 3–21 days in withdrawal. (B) Expression of DHPG-LTD in the presence of SKF 81297 was persistent across the withdrawal period. (C) SKF 81297 had no effect on D1 SPN firing of CIE mice across the withdrawal period. (D) DSE was increased in CIE mice across the withdrawal period.