FIGURE 4.

Expression of Bad signalling pathway–related proteins in renal I/R injury. Wild‐type and Fxr^−/− mice were subjected to 20 min of renal I/R, and kidneys were harvested at 24 h after surgery. (A) Quantitative analysis of the mouse apoptosis signalling pathway array in post‐I/R kidney. Histogram of quantitative analysis showing the different protein expression of the apoptotic markers between wild‐type and Fxr^−/− mice. (B) Representative WB images and expression ratio of p‐Bad to t‐Bad. (C) Representative WB images and expression ratio of p‐Akt to t‐Akt. (D) Representative WB images and (F) expression of p‐PI3K. (E) Representative WB images of Bcl‐2, Bcl‐xL and Bax. (G) Expression ratio of Bax to Bcl‐2. (H) Expression ratio of Bax to Bcl‐xL. The densities of bands were quantified with ImageJ analytical software. Each column represents the mean ± SD. *P < 0.05 and **P < 0.01 vs wild‐type mice at the same time point after I/R injury