FIGURE 2.

TSP50 binds directly to G6PD and co‐localizes with G6PD in the cytoplasm. A‐C, Huh7 cell was cultured and harvested, and after cell incubation with an anti‐TSP50 antibody, proteins binding to endogenous TSP50 were analysed by LC‐MS/MS. Candidate binding proteins to TSP50 were obtained after non‐specific proteins were removed. D and E, Huh7 cell and Bel7402 cell were harvested and subjected to immunoprecipitation with anti‐TSP50 antibody, followed by Western blot analysis with anti‐G6PD antibody. F and G, GST pull‐down of Flag‐G6PD by GST‐TSP50 using proteins purified in B21 bacteria, followed by Western blot analysis with anti‐G6PD and anti‐GST antibodies. H and I, Huh7 and Bel7402 cells were fixed and subjected to immunofluorescence analysis with anti‐TSP50 antibody or anti‐G6PD antibody for G6PD and TSP50 localization analysis