FIGURE 4.

K171 is a key site for TSP50 regulation of G6PD acetylation. A, Vectors encoding for multiple mutant G6PD were constructed using a site‐directed mutagenesis kit. B‐E, The effects of TSP50 on G6PD acetylation and enzyme activity were analysed by Western blot analysis and G6PD assay after pcDNA3.1‐TSP50 or shTSP50 transfection. F, Co‐immunoprecipitation was performed to analyse the effect of K171 and K403 site mutations on the binding of G6PD to KAT9 and SIRT2. G‐J, The level of G6PD K171 acetylation was detected using an anti‐G6PD K171ac antibody