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. 2021 Mar 31;54(3):176–181. doi: 10.5483/BMBRep.2021.54.3.170

Fig. 2.

Fig. 2

Single binding clusters of SRSF2/SRSF6 are not functional targets of SRSF2/SRSF6 proteins. (A) (Upper) Mutated sequences in SRSF2–M1, SRSF2–M2, SRSF2–M3, SRSF2–M4, SRSF2–M5 and SRSF2– M6 minigenes are shown. Potential SRSF2 binding sequences are shown in red. Mutated sequences are shown in green. (Lower) RT-PCR analysis of 5’ splice-site selection in Bcl-x mutant minigenes, including SRSF2–M1, SRSF2–M2, SRSF2–M3, SRSF2–M4, SRSF2–M5, and SRSF2–M6 minigenes are shown. Quantitation results are shown. (B) (Upper) Mutated sequences in SRSF6–M1, SRSF6–M2, SRSF6–M3, SRSF6–M4, SRSF6–M5, SRSF6–M6, and SRSF6–M7 minigenes are shown. Potential SRSF6 binding sequences are shown in blue. Mutated sequences are shown in green. (Lower) RT-PCR analysis of 5’ splice-site selection in Bcl-x mutant minigenes are shown. Quantitation results are shown.