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. 2021 Mar 31;54(3):176–181. doi: 10.5483/BMBRep.2021.54.3.170

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Copyright © 2021 by the The Korean Society for Biochemistry and Molecular Biology

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — Random deletion of exons did not disrupt functions of SRSF2 or SRSF6 in Bcl-x pre-mRNA splicing. (A) (Upper) Deleted regions from exon 2a in E2a/D1, E2a/D2, and E2a/D3 minigenes are shown with dot lined boxes. Length of the deleted parts are also shown. (Lower) RT-PCR analysis of 5’ splice-site selection in E2a/D1, E2a/D2 and E2a/D3 minigenes are shown. Quantitation results are shown. (B) (Upper) Deleted regions from exon 2b in E2b/D1, E2b/D2, E2b/D3 and E2b/D4 minigenes are shown with dot lined boxes. Length of the deleted parts are also shown. (Lower) RT-PCR analysis of 5’ splice-site selection in E2b/D1, E2b/D2, E2b/D3 and E2b/D4 minigenes are shown. Quantitation results are shown.