Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 4;40(13):2407–2421. doi: 10.1038/s41388-021-01718-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — A YAP1 signature was obtained from molecular signatures database of GSEA web site (http://software.broadinstitute.org/gsea/msigdb/index.jsp) and cross-referenced with the enzalutamide-resistant dataset (GSE52169). B, C YAP1 mRNA and protein were measured in parental LNCaP cells (WT) and enzalutamide-resistant cells (EnzaR) by RT-qPCR (n = 3) and western blot, respectively. Glucocorticoid receptor (GR) was used as positive marker for EnzaR cells. Asterisk indicates p < 0.05 using one-way ANOVA test. D YAP1 transcriptional activity was measured by transient transfection of 8xGIIC luciferase reporter into parental LNCaP cells (WT) and enzalutamide-resistant cells (EnzaR) (n = 3). Asterisk indicates p < 0.05 using one-way ANOVA test. E Several well-known YAP1 targets were assayed by RT-qPCR in parental LNCaP cells (WT) and enzalutamide-resistant cells (EnzaR) (n = 3). Asterisk indicates p < 0.05 using one-way ANOVA test. F YAP1 was knocked down in EnzaR cells by siRNA against YAP1 (40 nM). Results of western blot (upper panel) showed the knockdown efficiency of siYAP1 and cell proliferation (lower panel) was measured by MTS assay for indicated time point (n = 3). Asterisk indicates p < 0.05 using two-way ANOVA test. G Enzalutamide-resistant cells knocked down YAP1 expression by siRNA for 48 h were trypsinized and performed cell invasion assay for additional 16 h. Average of invaded cell numbers were counted from six different areas. Asterisk indicates p < 0.05 using two-tailed Student’s t-test.