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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Nature. 2021 Mar 10;592(7852):105–109. doi: 10.1038/s41586-021-03316-6

Extended Data Fig. 3|. Characterization of different lines of bbc/DEX::avrRpt2 plants.

Extended Data Fig. 3|

a, Schematic diagram of the experimental design. Leaf discs were first treated with flg22+DEX for 35min, and the production of ROS was detected by a microplate reader. Leaf discs were then washed with sterilized water for 4 times, 5min each time. Sterilized water (mock), 100nM flg22, 5μM DEX or 100nM flg22+5μM DEX was then added for detection of second-phase ROS. b, Expression levels of the avrRpt2 transgene in different transgenic lines 2h after infiltration with 5μM DEX. Statistical analysis was performed using one-way ANOVA with Tukey’s test. (mean ± s.e.m.; n = 3 (Col-0/DEX::avrRpt2 L2, bbc/DEX::avrRpt2 L1, bbc/DEX::avrRpt2 L2) or 4 (Col-0/DEX::avrRpt2 L1) biologically independent samples). Experiments were repeated three times with similar trends.