Extended Data Fig. 4|. AvrRpt2-triggered ETI-ROS depends on NADPH oxidase.

a-c, ROS production in Col-0/DEX::avrRpt2 L1 plants was inhibited by NADPH oxidase inhibitor DPI. Leaf discs were treated with 100nM flg22 and 5μM DEX. DPI, SHAM and NaN₃ were added at the beginning of measurement (mean ± s.e.m.; n (numbers of leaf disks) are indicated in the panel). b-c, Total photon counts are calculated from a at the PTI phase (0–30min) or ETI phase (60–200min). Statistical analysis was performed by one-way ANOVA with Tukey’s test. d, ETI-associated ROS burst is inhibited by DPI, an NADPH oxidase inhibitor. ROS was detected in Col-0/DEX::avrRpt2 plants after treatment of 100nM flg22 and 5μM DEX. Chemical inhibitors (DPI, SHAM or NaN₃) were added after the first ROS burst (about 40min after addition of flg22 and DEX). Data are displayed as mean ± s.e.m. n (numbers of leaf disks) are indicated in the panel. Box plots: centre line, median; box limits, lower and upper quartiles; dots, individual data points; whiskers, highest and lowest data points. Experiments in this figure were repeated three times with similar trends.