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. Author manuscript; available in PMC: 2021 Aug 15.
Published in final edited form as: Nat Neurosci. 2021 Feb 15;24(4):572–583. doi: 10.1038/s41593-020-00795-0

Extended Data Fig. 4. Vipr2 and Npas1 are novel, robust, and specific markers of α and γ motor neurons in the spinal cord.

Extended Data Fig. 4

a, Average expression of Vipr2 and Npas1 across all spinal cord cell populations (labeled), overlaid on UMAP. Arrow points to α and γ motor neuron clusters, respectively. b, Representative in situ hybridization against Chat/Rbfox3/Vipr2 in transverse spinal cord shows coexpression in the ventral horn (VH). n=4 biologically independent animals. Scale bar=100 µm. c, Representative in situ hybridization against Chat/Htr1d/Vipr2 in transverse spinal cord shows mutual exclusion. Scale bar=50 µm (inset) and 200 µm (overview). n=4 biologically independent animals. d, Representative in situ hybridization against Chat/Npas1/Rbfox3 in transverse spinal cord shows mutual exclusion of Rbfox3 and Npas1 in Chat+ cells. n=5 biologically independent animals. Scale bar=20 µm (inset) and 200 µm (overview). e, Representative in situ hybridization against Chat/Npas1/Vipr2 in transverse spinal cord shows mutual exclusion of novel markers Vipr2 and Npas1 in Chat+ cells. n=4 biologically independent animals. Scale bar=20 µm (inset) and 200 µm (overview).