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. 2021 Feb 7;26(3):195–208. doi: 10.1007/s10495-020-01655-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — HNK induces the paraptosis of NB4 cells. The NB4 cells were incubated with 0, 10, 20, and 30 μM HNK for 24 h. a The morphological changes of cells were observed under an optical microscope. b, c The Alix protein expression was analyzed by western blotting. d Real-Time PCR was performed to measure the mRNA level of Alix. The results are expressed as mean ± SD (n = 3). Compared with the solvent group, *P < 0.05, **P < 0.01