Fig. 7.

Inhibition of mTOR pathway can alleviate the generation of vacuolation. As shown in Fig. 6, the cells were divided into four groups. The cytoplasmic vacuolation (a, c, e) and endoplasmic reticulum expansion (yellow allows) (b, d, f) were observed under a microscope. g, h Endoplasmic reticulum stress proteins BiP and CHOP were analyzed by western blotting. i Western Blot analysis was used to measure the level of protein ubiquitination. The results are expressed as mean ± SD (n = 3). Compared with solvent group, ***P < 0.001