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. 2021 Apr 1;12(4):336. doi: 10.1038/s41419-021-03642-7

Fig. 3. RANK regulated CRC migration and invasion by activating ACP5 expression.

Fig. 3

a, b Western blot analysis of upregulation of ACP5 protein in RANK-overexpressing SW480 and Caco2 cells, and downregulation of ACP5 protein in RANK knockdown HT29 cells. c, f Western blotting showed that successful decreased ACP5 protein in RANK overexpression CRC cells transfected with siRNA-ACP5 and increased ACP5 protein by transfection with pFV-ACP5 plasmid in RANK knockdown CRC cells. d, e Knockdown of ACP5 inhibited RANK-induced migration and invasion in SW480RK and Caco2RK cells. Scales bars = 100 μm. g, h ACP5 overexpression increased migration and invasion of RANK-silencing HT29 cells. Scales bars = 100 μm. i Representative immunofluorescence of ACP5 distribution in high and low RANK expression CRC tissues. Nuclei (blue) were counterstained with DAPI. The white arrowheads indicate ACP5-positive cells are distributed within the tumor and stroma with high RANK expression. + (score 1–4), +++ (score 9–12). Scales bars = 10 μm. Data are mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.