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. 2021 Apr 1;11:7361. doi: 10.1038/s41598-021-86805-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of immunostaining for vimentin (Sertoli cells), DAZL (germ cells) and a marker of cellular proliferation (PCNA) in dog testes from different geographical locations. (a) Vimentin, (b) DAZL, (c) PCNA. Each point represents an individual testis from a specific geographical region: UK; West Midlands (WM: blue), South East (SE: green) East Midlands (EM: red) and Scandinavia; Vantaa, Finland (V: purple) and Copenhagen, Denmark (C: orange). Error bars and lines denote mean ± 1 S.E.M. Figures were created using GraphPad Prism version 8.0 for Mac, GraphPad Software, California, USA (https://www.graphpad.com).